Paternity Testing Research Today is a free monthly online journal that collates and summarizes the latest research about Paternity Testing, including details on accuracy, methods, procedure, dna testing.

Fluorescent multiplex amplification of three X-STR loci.

Liu QL, Lü DJ, Zhu JZ, Lu HL, Luo YM, Fang Q

Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China.

This study was carried out to evaluate the value of three X-STR loci (DXS6803, DXS981and DXS6809) in forensic application and thereby investigate their polymorphism. The primer for each locus was labeled with fluorochrome 6-FAM. A fluorescent multiplex PCR for simultaneously amplifying three X-STR loci was set up. The PCR products that were obtained were analyzed using capillary electrophoresis and ABI PRISM 3100 Genetic Analyzer, with GENESCAN Analysis Software. When 340 male and 195 female individuals of Han population in China were tested, 13, 12, and 11 alleles were observed for DXS6803, DXS981 and DXS6809, respectively. One hundred and eighty three haplotypes were detected in the male individuals. The haplotype diversity reached 0.9926. The results show that the three loci of the multiplex system provide significant information on polymorphism for forensic identification and paternity testing, particularly for complicated paternity deficient cases.

Published 22 December 2006 in Yi Chuan Xue Bao, 33(12): 1053-9.
Full-text of this article is available online (may require subscription).

© 2004-2008 Paternity Testing Research Today. All Rights Reserved.