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Allele Frequency of VNTR Locus D1S80 Observed in Denizli Province of Turkey.

Köseler A, Atalay A, Atalay EO

Department of Biophysics, Faculty of Medicine, Pamukkale University, Denizli, Turkey, akoseler@pau.edu.tr.

The variable numbers of tandem repeats (VNTR) locus D1S80, located on chromosome 1 (1p35-36), has a repeat unit 16 bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based on capillary electrophoresis separation to analyze D1S80 locus polymorphism among 74 subjects from Denizli province, Turkey, finding an amplified fragment length size of 379-635 bp. Allele repeat numbers were deduced from these sizes and sequence comparison. The most common alleles were repeat units 24 (34.3%) and 18 (22.4%), with frequencies of 0.414 and 0.207, respectively. Other alleles were 25 (7.86%), 28 (5.71%), 22 (4.25%), and 29 (2.86%). The allele with 23 repeat units was not observed. Results were in Hardy-Weinberg linkage disequilibrium. Observed heterozygosity was 0.614, and expected heterozygosity was 0.787. Theta(k) value was 4.86 (95% confidence interval limits). Capillary electrophoresis is a powerful approach for accurate identification of VNTR loci, especially for low base pair units like D1S80, for prenatal diagnosis, linkage analysis, forensic identification, paternity testing, anthropological research, and phylogenetic studies.

Published 8 June 2009 in Biochem Genet.
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Articles on Paternity Testing published 4 June 2009:

From here to paternity: Neural correlates of the onset of paternal behavior in California mice (Peromyscus californicus).   Horm Behav.

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Articles on Paternity Testing published 1 June 2009:

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Articles on Paternity Testing published 15 May 2009:

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Articles on Paternity Testing published 7 May 2009:

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Articles on Paternity Testing published 30 March 2009:

Sequence alterations in the YBX2 gene are associated with male factor infertility.   Fertil Steril, 91(4): 1090-5.

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Articles on Paternity Testing published 27 March 2009:

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Articles on Paternity Testing published 24 March 2009:

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